Clinical testing with this and other inhibitors will be required to relate potency, duration of pharmacokineticpharmacodynamic effect, and the spectrum of enzymatic selectivity to the safety and efficacy profile that provides optimal benefit for 5-hydroxytryptophan Cancer patients.PHARMACOLOGICAL CHARACTERIZATION OF CP. Click on Request Permissions which will take you to the Copyright Clearance Centers Downloaded from cancerres.aacrjournals.org on April. American Association for Cancer Research. Bortezomib is a potent and specific inhibitor of the S proteasome that is currently in clinical trials for treatment of various malignancies, including prostate cancer.Bortezomib induced strong stabilization of p, but it did not promote phosphorylation on serines and, and p remained bound to its inhibitor, mdm.Nonetheless, bortezomib stimulated p translocation to the nucleus and enhanced p DNA binding, accumulation of pdependent transcripts, and activation of a presponsive reporter gene.Together, our data demonstrate that bortezomib stimulates p activation via a novel mechanism.All conventional therapies eventually fail if the disease has progressed beyond the confines of the original organ. Thus, there is currently an aggressive search for new therapeutic strategies to Riboflavin combat advanced disease.The S proteasome is responsible for ubiquitinmediated protein degradation in eukaryotes, and it has recently gained attention as a therapeutic target in cancer is the first and currently only proteasome inhibitor to undergo clinical trials for several forms of cancer. The therapeutic potential of the drug is thought to lie in its ability to cause the stabilization of proteins that inhibit cell survival and cell cycle progression. In quiescence, p has a very short halflife, and, by way of its ubiquitin ligase function, targets the tumor suppressor for degradation by the S proteasome.Cellular stress can lead to a series of posttranslational modifications of p that result in its activation. These changes, including a series of phosphorylation events, cause p to stabilize by disassociating from mdm, tetramerize, localize to the nucleus, and function primarily as a regulator of transcription. The consequences of p activation include cell cycle arrest at the G and G checkpoints, activation of the DNA repair machinery, and, if repair is unsuccessful, the initiation of apoptosis. The costs of publication of this article were defrayed in part by the payment of page charges.We designed the experiments to test several biochemical parameters normally associated with p activation, including serine phosphorylation, association with mdm, and nuclear localization.We also performed direct analyses of the DNA binding and transcriptional transactivation functions of p in cells exposed to the drug.Together, our data demonstrate that bortezomib promotes p activation via a novel mechanism.Lysates were transferred to microcentrifuge tubes and centrifuged at C for min at, g.Supernatants were transferred to new tubes, and g samples were mixed with equal volumes of SDSPAGE sample buffer. Samples were boiled, centrifuged briefly, and resolved by SDSPAGE. Membranes were blocked in TBST and skim milk for h.Blots were washed briefly in TBST before incubation with primary antibodies specific for p or mdm diluted in TBST containing milk.Blots were gently shaken overnight at C and washed in TBST before incubation with appropriate secondary antibodies horseradish peroxidaseconjugated sheep antimouse or donkey antirabbit antibody and developed by enhanced chemiluminescence.